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GC content and Size of sequenced genomes on NCBI
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| "_uuid": "847638d7b6aaeaa56c7032d212ad9fb98f6dc4c6" | |
| }, | |
| "source": [ | |
| "# A visual exploration of the size and GC content of sequenced genomes avaliable on [NCBI](https://www.ncbi.nlm.nih.gov/)\n", | |
| "\n", | |
| "Genome size is commonly reported in megabase pairs (megabase = 1,000,000 base pairs of DNA). The size of genomes is extremly variable across species and influenced by a large number of factors (if you're curious the human genome is ~3,000 Mb (megabase pairs), or 3 billion base pairs in size). The NCBI sequenced genome dataset includes genomes from three domains of organisms: eukaryotes (multi cellular organisms, like you, ducks and ferms), porkaryote (single cell, bacteria) and virus genomes (not a Linnean doman... [more a weird grey area](https://www.scientificamerican.com/article/are-viruses-alive-2004/). Here I plot the size of the genomes in these three domains to compare them, and also look at the GC content of the genomes across these domains. %GC content is the percentage of DNA base pairs composed of G and C (guanine and cytosine) GC-content is indicative of many protein coding genes, and the %GC content is indicative of a gene-rich genome. GC ratios within a genome are know to be extremly variable, with low %GC indicating a large amount of non-coding DNA in the genome. \n", | |
| "\n", | |
| "These data are derived from [NCBI's repository of sequenced genomes](https://www.ncbi.nlm.nih.gov/home/genomes/). Through the links provided in the Kaggle dataset, one can access the entire sequence to any of these genomes as they are pubically avaliable (for free! thanks science!)" | |
| ] | |
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| "source": [ | |
| "## Loading the data, assessment and cleanup" | |
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| "── Attaching packages ─────────────────────────────────────── tidyverse 1.2.1 ──\n", | |
| "✔ ggplot2 2.2.1 ✔ purrr 0.2.4\n", | |
| "✔ tibble 1.3.4 ✔ dplyr 0.7.4\n", | |
| "✔ tidyr 0.7.2 ✔ stringr 1.2.0\n", | |
| "✔ readr 1.1.1 ✔ forcats 0.2.0\n", | |
| "── Conflicts ────────────────────────────────────────── tidyverse_conflicts() ──\n", | |
| "✖ dplyr::filter() masks stats::filter()\n", | |
| "✖ dplyr::lag() masks stats::lag()\n" | |
| ] | |
| } | |
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| "source": [ | |
| "library('tidyverse')\n", | |
| "library('ggthemes')" | |
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| { | |
| "cell_type": "code", | |
| "execution_count": 2, | |
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| "scrolled": true | |
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| "name": "stderr", | |
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| "text": [ | |
| "Parsed with column specification:\n", | |
| "cols(\n", | |
| " `#Organism Name` = col_character(),\n", | |
| " `Organism Groups` = col_character(),\n", | |
| " Strain = col_character(),\n", | |
| " BioSample = col_character(),\n", | |
| " BioProject = col_character(),\n", | |
| " Assembly = col_character(),\n", | |
| " Level = col_character(),\n", | |
| " `Size(Mb)` = col_double(),\n", | |
| " `GC%` = col_double(),\n", | |
| " Replicons = col_character(),\n", | |
| " WGS = col_character(),\n", | |
| " Scaffolds = col_integer(),\n", | |
| " CDS = col_integer(),\n", | |
| " `Release Date` = col_datetime(format = \"\"),\n", | |
| " `GenBank FTP` = col_character(),\n", | |
| " `RefSeq FTP` = col_character()\n", | |
| ")\n" | |
| ] | |
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| { | |
| "data": { | |
| "text/html": [ | |
| "<table>\n", | |
| "<thead><tr><th scope=col>#Organism Name</th><th scope=col>Organism Groups</th><th scope=col>Strain</th><th scope=col>BioSample</th><th scope=col>BioProject</th><th scope=col>Assembly</th><th scope=col>Level</th><th scope=col>Size(Mb)</th><th scope=col>GC%</th><th scope=col>Replicons</th><th scope=col>WGS</th><th scope=col>Scaffolds</th><th scope=col>CDS</th><th scope=col>Release Date</th><th scope=col>GenBank FTP</th><th scope=col>RefSeq FTP</th></tr></thead>\n", | |
| "<tbody>\n", | |
| "\t<tr><td>Emiliania huxleyi CCMP1516 </td><td>Eukaryota;Protists;Other Protists </td><td>CCMP1516 </td><td>SAMN02744062 </td><td>PRJNA77753 </td><td>GCA_000372725.1 </td><td>Scaffold </td><td> 167.676 </td><td>64.5000 </td><td>NA </td><td>AHAL01 </td><td> 7795 </td><td>38554 </td><td>2013-04-19 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 </td></tr>\n", | |
| "\t<tr><td>Arabidopsis thaliana </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN03081427 </td><td>PRJNA10719 </td><td>GCA_000001735.1 </td><td>Chromosome </td><td> 119.668 </td><td>36.0528 </td><td>chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 </td><td>NA </td><td> 7 </td><td>48350 </td><td>2001-08-13 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 </td></tr>\n", | |
| "\t<tr><td>Medicago truncatula </td><td>Eukaryota;Plants;Land Plants </td><td>A17 </td><td>SAMN02299339 </td><td>PRJNA10791 </td><td>GCA_000219495.2 </td><td>Chromosome </td><td> 412.924 </td><td>34.0470 </td><td>chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 </td><td>APNO01 </td><td> 2187 </td><td>57661 </td><td>2011-08-12 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 </td></tr>\n", | |
| "\t<tr><td>Glycine max </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN00002965 </td><td>PRJNA19861 </td><td>GCA_000004515.3 </td><td>Chromosome </td><td> 978.972 </td><td>35.1208 </td><td>chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1</td><td>ACUP02 </td><td> 1191 </td><td>71525 </td><td>2010-01-05 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 </td></tr>\n", | |
| "\t<tr><td>Solanum lycopersicum </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN02981290 </td><td>PRJNA119 </td><td>GCA_000188115.2 </td><td>Chromosome </td><td> 823.786 </td><td>35.7097 </td><td>chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 </td><td>AEKE02 </td><td> 3224 </td><td>36010 </td><td>2010-12-10 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 </td></tr>\n", | |
| "\t<tr><td>Hordeum vulgare </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMEA3903302 </td><td>PRJEB13020 </td><td>GCA_900075435.2 </td><td>Scaffold </td><td>9788.860 </td><td> 0.0000 </td><td>NA </td><td>FJWB02 </td><td>72295 </td><td> 0 </td><td>2016-08-25 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 </td><td>NA </td></tr>\n", | |
| "</tbody>\n", | |
| "</table>\n" | |
| ], | |
| "text/latex": [ | |
| "\\begin{tabular}{r|llllllllllllllll}\n", | |
| " \\#Organism Name & Organism Groups & Strain & BioSample & BioProject & Assembly & Level & Size(Mb) & GC\\% & Replicons & WGS & Scaffolds & CDS & Release Date & GenBank FTP & RefSeq FTP\\\\\n", | |
| "\\hline\n", | |
| "\t Emiliania huxleyi CCMP1516 & Eukaryota;Protists;Other Protists & CCMP1516 & SAMN02744062 & PRJNA77753 & GCA\\_000372725.1 & Scaffold & 167.676 & 64.5000 & NA & AHAL01 & 7795 & 38554 & 2013-04-19 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA\\_000372725.1\\_Emiliana\\_huxleyi\\_CCMP1516\\_main\\_genome\\_assembly\\_v1.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF\\_000372725.1\\_Emiliana\\_huxleyi\\_CCMP1516\\_main\\_genome\\_assembly\\_v1.0 \\\\\n", | |
| "\t Arabidopsis thaliana & Eukaryota;Plants;Land Plants & NA & SAMN03081427 & PRJNA10719 & GCA\\_000001735.1 & Chromosome & 119.668 & 36.0528 & chromosome 1:NC\\_003070.9/CP002684.1; chromosome 2:NC\\_003071.7/CP002685.1; chromosome 3:NC\\_003074.8/CP002686.1; chromosome 4:NC\\_003075.7/CP002687.1; chromosome 5:NC\\_003076.8/CP002688.1; mitochondrion MT:NC\\_001284.2/Y08501.2; chloroplast Pltd:NC\\_000932.1/AP000423.1 & NA & 7 & 48350 & 2001-08-13 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA\\_000001735.1\\_TAIR10 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF\\_000001735.3\\_TAIR10 \\\\\n", | |
| "\t Medicago truncatula & Eukaryota;Plants;Land Plants & A17 & SAMN02299339 & PRJNA10791 & GCA\\_000219495.2 & Chromosome & 412.924 & 34.0470 & chromosome 1:NC\\_016407.2/CM001217.2; chromosome 2:NC\\_016408.2/CM001218.2; chromosome 3:NC\\_016409.2/CM001219.2; chromosome 4:NC\\_016410.2/CM001220.2; chromosome 5:NC\\_016411.2/CM001221.2; chromosome 6:NC\\_016412.2/CM001222.2; chromosome 7:NC\\_016413.2/CM001223.2; chromosome 8:NC\\_016414.2/CM001224.2; chloroplast Pltd:NC\\_003119.6/AC093544.8 & APNO01 & 2187 & 57661 & 2011-08-12 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA\\_000219495.2\\_MedtrA17\\_4.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF\\_000219495.3\\_MedtrA17\\_4.0 \\\\\n", | |
| "\t Glycine max & Eukaryota;Plants;Land Plants & NA & SAMN00002965 & PRJNA19861 & GCA\\_000004515.3 & Chromosome & 978.972 & 35.1208 & chromosome 1:NC\\_016088.2/CM000834.2; chromosome 2:NC\\_016089.2/CM000835.2; chromosome 3:NC\\_016090.2/CM000836.2; chromosome 4:NC\\_016091.2/CM000837.2; chromosome 5:NC\\_016092.2/CM000838.2; chromosome 6:NC\\_016093.2/CM000839.2; chromosome 7:NC\\_016094.2/CM000840.2; chromosome 8:NC\\_016095.2/CM000841.2; chromosome 9:NC\\_016096.2/CM000842.2; chromosome 10:NC\\_016097.2/CM000843.2; chromosome 11:NC\\_016098.2/CM000844.2; chromosome 12:NC\\_016099.2/CM000845.2; chromosome 13:NC\\_016100.2/CM000846.2; chromosome 14:NC\\_016101.2/CM000847.2; chromosome 15:NC\\_016102.2/CM000848.2; chromosome 16:NC\\_016103.2/CM000849.2; chromosome 17:NC\\_016104.2/CM000850.2; chromosome 18:NC\\_016105.2/CM000851.2; chromosome 19:NC\\_016106.2/CM000852.2; chromosome 20:NC\\_016107.2/CM000853.2; chloroplast Pltd:NC\\_007942.1/DQ317523.1; mitochondrion MT:NC\\_020455.1/JX463295.1 & ACUP02 & 1191 & 71525 & 2010-01-05 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA\\_000004515.3\\_Glycine\\_max\\_v2.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF\\_000004515.4\\_Glycine\\_max\\_v2.0 \\\\\n", | |
| "\t Solanum lycopersicum & Eukaryota;Plants;Land Plants & NA & SAMN02981290 & PRJNA119 & GCA\\_000188115.2 & Chromosome & 823.786 & 35.7097 & chromosome 1:NC\\_015438.2/CM001064.2; chromosome 2:NC\\_015439.2/CM001065.2; chromosome 3:NC\\_015440.2/CM001066.2; chromosome 4:NC\\_015441.2/CM001067.2; chromosome 5:NC\\_015442.2/CM001068.2; chromosome 6:NC\\_015443.2/CM001069.2; chromosome 7:NC\\_015444.2/CM001070.2; chromosome 8:NC\\_015445.2/CM001071.2; chromosome 9:NC\\_015446.2/CM001072.2; chromosome 10:NC\\_015447.2/CM001073.2; chromosome 11:NC\\_015448.2/CM001074.2; chromosome 12:NC\\_015449.2/CM001075.2; chloroplast:NC\\_007898.3/DQ347959.1 & AEKE02 & 3224 & 36010 & 2010-12-10 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA\\_000188115.2\\_SL2.50 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF\\_000188115.3\\_SL2.50 \\\\\n", | |
| "\t Hordeum vulgare & Eukaryota;Plants;Land Plants & NA & SAMEA3903302 & PRJEB13020 & GCA\\_900075435.2 & Scaffold & 9788.860 & 0.0000 & NA & FJWB02 & 72295 & 0 & 2016-08-25 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA\\_900075435.2\\_barley\\_BACs\\_2 & NA \\\\\n", | |
| "\\end{tabular}\n" | |
| ], | |
| "text/markdown": [ | |
| "\n", | |
| "#Organism Name | Organism Groups | Strain | BioSample | BioProject | Assembly | Level | Size(Mb) | GC% | Replicons | WGS | Scaffolds | CDS | Release Date | GenBank FTP | RefSeq FTP | \n", | |
| "|---|---|---|---|---|---|\n", | |
| "| Emiliania huxleyi CCMP1516 | Eukaryota;Protists;Other Protists | CCMP1516 | SAMN02744062 | PRJNA77753 | GCA_000372725.1 | Scaffold | 167.676 | 64.5000 | NA | AHAL01 | 7795 | 38554 | 2013-04-19 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 | \n", | |
| "| Arabidopsis thaliana | Eukaryota;Plants;Land Plants | NA | SAMN03081427 | PRJNA10719 | GCA_000001735.1 | Chromosome | 119.668 | 36.0528 | chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 | NA | 7 | 48350 | 2001-08-13 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 | \n", | |
| "| Medicago truncatula | Eukaryota;Plants;Land Plants | A17 | SAMN02299339 | PRJNA10791 | GCA_000219495.2 | Chromosome | 412.924 | 34.0470 | chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 | APNO01 | 2187 | 57661 | 2011-08-12 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 | \n", | |
| "| Glycine max | Eukaryota;Plants;Land Plants | NA | SAMN00002965 | PRJNA19861 | GCA_000004515.3 | Chromosome | 978.972 | 35.1208 | chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1 | ACUP02 | 1191 | 71525 | 2010-01-05 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 | \n", | |
| "| Solanum lycopersicum | Eukaryota;Plants;Land Plants | NA | SAMN02981290 | PRJNA119 | GCA_000188115.2 | Chromosome | 823.786 | 35.7097 | chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 | AEKE02 | 3224 | 36010 | 2010-12-10 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 | \n", | |
| "| Hordeum vulgare | Eukaryota;Plants;Land Plants | NA | SAMEA3903302 | PRJEB13020 | GCA_900075435.2 | Scaffold | 9788.860 | 0.0000 | NA | FJWB02 | 72295 | 0 | 2016-08-25 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 | NA | \n", | |
| "\n", | |
| "\n" | |
| ], | |
| "text/plain": [ | |
| " #Organism Name Organism Groups Strain \n", | |
| "1 Emiliania huxleyi CCMP1516 Eukaryota;Protists;Other Protists CCMP1516\n", | |
| "2 Arabidopsis thaliana Eukaryota;Plants;Land Plants NA \n", | |
| "3 Medicago truncatula Eukaryota;Plants;Land Plants A17 \n", | |
| "4 Glycine max Eukaryota;Plants;Land Plants NA \n", | |
| "5 Solanum lycopersicum Eukaryota;Plants;Land Plants NA \n", | |
| "6 Hordeum vulgare Eukaryota;Plants;Land Plants NA \n", | |
| " BioSample BioProject Assembly Level Size(Mb) GC% \n", | |
| "1 SAMN02744062 PRJNA77753 GCA_000372725.1 Scaffold 167.676 64.5000\n", | |
| "2 SAMN03081427 PRJNA10719 GCA_000001735.1 Chromosome 119.668 36.0528\n", | |
| "3 SAMN02299339 PRJNA10791 GCA_000219495.2 Chromosome 412.924 34.0470\n", | |
| "4 SAMN00002965 PRJNA19861 GCA_000004515.3 Chromosome 978.972 35.1208\n", | |
| "5 SAMN02981290 PRJNA119 GCA_000188115.2 Chromosome 823.786 35.7097\n", | |
| "6 SAMEA3903302 PRJEB13020 GCA_900075435.2 Scaffold 9788.860 0.0000\n", | |
| " Replicons \n", | |
| "1 NA \n", | |
| "2 chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 \n", | |
| "3 chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 \n", | |
| "4 chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1\n", | |
| "5 chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 \n", | |
| "6 NA \n", | |
| " WGS Scaffolds CDS Release Date\n", | |
| "1 AHAL01 7795 38554 2013-04-19 \n", | |
| "2 NA 7 48350 2001-08-13 \n", | |
| "3 APNO01 2187 57661 2011-08-12 \n", | |
| "4 ACUP02 1191 71525 2010-01-05 \n", | |
| "5 AEKE02 3224 36010 2010-12-10 \n", | |
| "6 FJWB02 72295 0 2016-08-25 \n", | |
| " GenBank FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0\n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 \n", | |
| "4 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 \n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 \n", | |
| "6 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 \n", | |
| " RefSeq FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0\n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 \n", | |
| "4 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 \n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 \n", | |
| "6 NA " | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "raw_eukaryote = read_csv('all_genomes_lists/eukaryotes.csv')\n", | |
| "head(raw_eukaryote)" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 3, | |
| "metadata": { | |
| "_cell_guid": "a527a8b0-86d5-4481-8b8a-0e6716f65d6d", | |
| "_uuid": "4fd42e5d21225b141111471c5a2346032e28f671", | |
| "scrolled": true | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "<table>\n", | |
| "<thead><tr><th scope=col>#Organism Name</th><th scope=col>Organism Groups</th><th scope=col>Strain</th><th scope=col>BioSample</th><th scope=col>BioProject</th><th scope=col>Assembly</th><th scope=col>Level</th><th scope=col>Size(Mb)</th><th scope=col>GC%</th><th scope=col>Replicons</th><th scope=col>WGS</th><th scope=col>Scaffolds</th><th scope=col>CDS</th><th scope=col>Release Date</th><th scope=col>GenBank FTP</th><th scope=col>RefSeq FTP</th></tr></thead>\n", | |
| "<tbody>\n", | |
| "\t<tr><td>Emiliania huxleyi CCMP1516 </td><td>Eukaryota;Protists;Other Protists </td><td>CCMP1516 </td><td>SAMN02744062 </td><td>PRJNA77753 </td><td>GCA_000372725.1 </td><td>Scaffold </td><td> 167.676 </td><td>64.5000 </td><td>NA </td><td>AHAL01 </td><td> 7795 </td><td>38554 </td><td>2013-04-19 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 </td></tr>\n", | |
| "\t<tr><td>Arabidopsis thaliana </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN03081427 </td><td>PRJNA10719 </td><td>GCA_000001735.1 </td><td>Chromosome </td><td> 119.668 </td><td>36.0528 </td><td>chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 </td><td>NA </td><td> 7 </td><td>48350 </td><td>2001-08-13 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 </td></tr>\n", | |
| "\t<tr><td>Medicago truncatula </td><td>Eukaryota;Plants;Land Plants </td><td>A17 </td><td>SAMN02299339 </td><td>PRJNA10791 </td><td>GCA_000219495.2 </td><td>Chromosome </td><td> 412.924 </td><td>34.0470 </td><td>chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 </td><td>APNO01 </td><td> 2187 </td><td>57661 </td><td>2011-08-12 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 </td></tr>\n", | |
| "\t<tr><td>Glycine max </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN00002965 </td><td>PRJNA19861 </td><td>GCA_000004515.3 </td><td>Chromosome </td><td> 978.972 </td><td>35.1208 </td><td>chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1</td><td>ACUP02 </td><td> 1191 </td><td>71525 </td><td>2010-01-05 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 </td></tr>\n", | |
| "\t<tr><td>Solanum lycopersicum </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMN02981290 </td><td>PRJNA119 </td><td>GCA_000188115.2 </td><td>Chromosome </td><td> 823.786 </td><td>35.7097 </td><td>chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 </td><td>AEKE02 </td><td> 3224 </td><td>36010 </td><td>2010-12-10 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 </td></tr>\n", | |
| "\t<tr><td>Hordeum vulgare </td><td>Eukaryota;Plants;Land Plants </td><td>NA </td><td>SAMEA3903302 </td><td>PRJEB13020 </td><td>GCA_900075435.2 </td><td>Scaffold </td><td>9788.860 </td><td> 0.0000 </td><td>NA </td><td>FJWB02 </td><td>72295 </td><td> 0 </td><td>2016-08-25 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 </td><td>NA </td></tr>\n", | |
| "</tbody>\n", | |
| "</table>\n" | |
| ], | |
| "text/latex": [ | |
| "\\begin{tabular}{r|llllllllllllllll}\n", | |
| " \\#Organism Name & Organism Groups & Strain & BioSample & BioProject & Assembly & Level & Size(Mb) & GC\\% & Replicons & WGS & Scaffolds & CDS & Release Date & GenBank FTP & RefSeq FTP\\\\\n", | |
| "\\hline\n", | |
| "\t Emiliania huxleyi CCMP1516 & Eukaryota;Protists;Other Protists & CCMP1516 & SAMN02744062 & PRJNA77753 & GCA\\_000372725.1 & Scaffold & 167.676 & 64.5000 & NA & AHAL01 & 7795 & 38554 & 2013-04-19 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA\\_000372725.1\\_Emiliana\\_huxleyi\\_CCMP1516\\_main\\_genome\\_assembly\\_v1.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF\\_000372725.1\\_Emiliana\\_huxleyi\\_CCMP1516\\_main\\_genome\\_assembly\\_v1.0 \\\\\n", | |
| "\t Arabidopsis thaliana & Eukaryota;Plants;Land Plants & NA & SAMN03081427 & PRJNA10719 & GCA\\_000001735.1 & Chromosome & 119.668 & 36.0528 & chromosome 1:NC\\_003070.9/CP002684.1; chromosome 2:NC\\_003071.7/CP002685.1; chromosome 3:NC\\_003074.8/CP002686.1; chromosome 4:NC\\_003075.7/CP002687.1; chromosome 5:NC\\_003076.8/CP002688.1; mitochondrion MT:NC\\_001284.2/Y08501.2; chloroplast Pltd:NC\\_000932.1/AP000423.1 & NA & 7 & 48350 & 2001-08-13 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA\\_000001735.1\\_TAIR10 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF\\_000001735.3\\_TAIR10 \\\\\n", | |
| "\t Medicago truncatula & Eukaryota;Plants;Land Plants & A17 & SAMN02299339 & PRJNA10791 & GCA\\_000219495.2 & Chromosome & 412.924 & 34.0470 & chromosome 1:NC\\_016407.2/CM001217.2; chromosome 2:NC\\_016408.2/CM001218.2; chromosome 3:NC\\_016409.2/CM001219.2; chromosome 4:NC\\_016410.2/CM001220.2; chromosome 5:NC\\_016411.2/CM001221.2; chromosome 6:NC\\_016412.2/CM001222.2; chromosome 7:NC\\_016413.2/CM001223.2; chromosome 8:NC\\_016414.2/CM001224.2; chloroplast Pltd:NC\\_003119.6/AC093544.8 & APNO01 & 2187 & 57661 & 2011-08-12 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA\\_000219495.2\\_MedtrA17\\_4.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF\\_000219495.3\\_MedtrA17\\_4.0 \\\\\n", | |
| "\t Glycine max & Eukaryota;Plants;Land Plants & NA & SAMN00002965 & PRJNA19861 & GCA\\_000004515.3 & Chromosome & 978.972 & 35.1208 & chromosome 1:NC\\_016088.2/CM000834.2; chromosome 2:NC\\_016089.2/CM000835.2; chromosome 3:NC\\_016090.2/CM000836.2; chromosome 4:NC\\_016091.2/CM000837.2; chromosome 5:NC\\_016092.2/CM000838.2; chromosome 6:NC\\_016093.2/CM000839.2; chromosome 7:NC\\_016094.2/CM000840.2; chromosome 8:NC\\_016095.2/CM000841.2; chromosome 9:NC\\_016096.2/CM000842.2; chromosome 10:NC\\_016097.2/CM000843.2; chromosome 11:NC\\_016098.2/CM000844.2; chromosome 12:NC\\_016099.2/CM000845.2; chromosome 13:NC\\_016100.2/CM000846.2; chromosome 14:NC\\_016101.2/CM000847.2; chromosome 15:NC\\_016102.2/CM000848.2; chromosome 16:NC\\_016103.2/CM000849.2; chromosome 17:NC\\_016104.2/CM000850.2; chromosome 18:NC\\_016105.2/CM000851.2; chromosome 19:NC\\_016106.2/CM000852.2; chromosome 20:NC\\_016107.2/CM000853.2; chloroplast Pltd:NC\\_007942.1/DQ317523.1; mitochondrion MT:NC\\_020455.1/JX463295.1 & ACUP02 & 1191 & 71525 & 2010-01-05 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA\\_000004515.3\\_Glycine\\_max\\_v2.0 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF\\_000004515.4\\_Glycine\\_max\\_v2.0 \\\\\n", | |
| "\t Solanum lycopersicum & Eukaryota;Plants;Land Plants & NA & SAMN02981290 & PRJNA119 & GCA\\_000188115.2 & Chromosome & 823.786 & 35.7097 & chromosome 1:NC\\_015438.2/CM001064.2; chromosome 2:NC\\_015439.2/CM001065.2; chromosome 3:NC\\_015440.2/CM001066.2; chromosome 4:NC\\_015441.2/CM001067.2; chromosome 5:NC\\_015442.2/CM001068.2; chromosome 6:NC\\_015443.2/CM001069.2; chromosome 7:NC\\_015444.2/CM001070.2; chromosome 8:NC\\_015445.2/CM001071.2; chromosome 9:NC\\_015446.2/CM001072.2; chromosome 10:NC\\_015447.2/CM001073.2; chromosome 11:NC\\_015448.2/CM001074.2; chromosome 12:NC\\_015449.2/CM001075.2; chloroplast:NC\\_007898.3/DQ347959.1 & AEKE02 & 3224 & 36010 & 2010-12-10 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA\\_000188115.2\\_SL2.50 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF\\_000188115.3\\_SL2.50 \\\\\n", | |
| "\t Hordeum vulgare & Eukaryota;Plants;Land Plants & NA & SAMEA3903302 & PRJEB13020 & GCA\\_900075435.2 & Scaffold & 9788.860 & 0.0000 & NA & FJWB02 & 72295 & 0 & 2016-08-25 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA\\_900075435.2\\_barley\\_BACs\\_2 & NA \\\\\n", | |
| "\\end{tabular}\n" | |
| ], | |
| "text/markdown": [ | |
| "\n", | |
| "#Organism Name | Organism Groups | Strain | BioSample | BioProject | Assembly | Level | Size(Mb) | GC% | Replicons | WGS | Scaffolds | CDS | Release Date | GenBank FTP | RefSeq FTP | \n", | |
| "|---|---|---|---|---|---|\n", | |
| "| Emiliania huxleyi CCMP1516 | Eukaryota;Protists;Other Protists | CCMP1516 | SAMN02744062 | PRJNA77753 | GCA_000372725.1 | Scaffold | 167.676 | 64.5000 | NA | AHAL01 | 7795 | 38554 | 2013-04-19 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0 | \n", | |
| "| Arabidopsis thaliana | Eukaryota;Plants;Land Plants | NA | SAMN03081427 | PRJNA10719 | GCA_000001735.1 | Chromosome | 119.668 | 36.0528 | chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 | NA | 7 | 48350 | 2001-08-13 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 | \n", | |
| "| Medicago truncatula | Eukaryota;Plants;Land Plants | A17 | SAMN02299339 | PRJNA10791 | GCA_000219495.2 | Chromosome | 412.924 | 34.0470 | chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 | APNO01 | 2187 | 57661 | 2011-08-12 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 | \n", | |
| "| Glycine max | Eukaryota;Plants;Land Plants | NA | SAMN00002965 | PRJNA19861 | GCA_000004515.3 | Chromosome | 978.972 | 35.1208 | chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1 | ACUP02 | 1191 | 71525 | 2010-01-05 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 | \n", | |
| "| Solanum lycopersicum | Eukaryota;Plants;Land Plants | NA | SAMN02981290 | PRJNA119 | GCA_000188115.2 | Chromosome | 823.786 | 35.7097 | chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 | AEKE02 | 3224 | 36010 | 2010-12-10 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 | \n", | |
| "| Hordeum vulgare | Eukaryota;Plants;Land Plants | NA | SAMEA3903302 | PRJEB13020 | GCA_900075435.2 | Scaffold | 9788.860 | 0.0000 | NA | FJWB02 | 72295 | 0 | 2016-08-25 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 | NA | \n", | |
| "\n", | |
| "\n" | |
| ], | |
| "text/plain": [ | |
| " #Organism Name Organism Groups Strain \n", | |
| "1 Emiliania huxleyi CCMP1516 Eukaryota;Protists;Other Protists CCMP1516\n", | |
| "2 Arabidopsis thaliana Eukaryota;Plants;Land Plants NA \n", | |
| "3 Medicago truncatula Eukaryota;Plants;Land Plants A17 \n", | |
| "4 Glycine max Eukaryota;Plants;Land Plants NA \n", | |
| "5 Solanum lycopersicum Eukaryota;Plants;Land Plants NA \n", | |
| "6 Hordeum vulgare Eukaryota;Plants;Land Plants NA \n", | |
| " BioSample BioProject Assembly Level Size(Mb) GC% \n", | |
| "1 SAMN02744062 PRJNA77753 GCA_000372725.1 Scaffold 167.676 64.5000\n", | |
| "2 SAMN03081427 PRJNA10719 GCA_000001735.1 Chromosome 119.668 36.0528\n", | |
| "3 SAMN02299339 PRJNA10791 GCA_000219495.2 Chromosome 412.924 34.0470\n", | |
| "4 SAMN00002965 PRJNA19861 GCA_000004515.3 Chromosome 978.972 35.1208\n", | |
| "5 SAMN02981290 PRJNA119 GCA_000188115.2 Chromosome 823.786 35.7097\n", | |
| "6 SAMEA3903302 PRJEB13020 GCA_900075435.2 Scaffold 9788.860 0.0000\n", | |
| " Replicons \n", | |
| "1 NA \n", | |
| "2 chromosome 1:NC_003070.9/CP002684.1; chromosome 2:NC_003071.7/CP002685.1; chromosome 3:NC_003074.8/CP002686.1; chromosome 4:NC_003075.7/CP002687.1; chromosome 5:NC_003076.8/CP002688.1; mitochondrion MT:NC_001284.2/Y08501.2; chloroplast Pltd:NC_000932.1/AP000423.1 \n", | |
| "3 chromosome 1:NC_016407.2/CM001217.2; chromosome 2:NC_016408.2/CM001218.2; chromosome 3:NC_016409.2/CM001219.2; chromosome 4:NC_016410.2/CM001220.2; chromosome 5:NC_016411.2/CM001221.2; chromosome 6:NC_016412.2/CM001222.2; chromosome 7:NC_016413.2/CM001223.2; chromosome 8:NC_016414.2/CM001224.2; chloroplast Pltd:NC_003119.6/AC093544.8 \n", | |
| "4 chromosome 1:NC_016088.2/CM000834.2; chromosome 2:NC_016089.2/CM000835.2; chromosome 3:NC_016090.2/CM000836.2; chromosome 4:NC_016091.2/CM000837.2; chromosome 5:NC_016092.2/CM000838.2; chromosome 6:NC_016093.2/CM000839.2; chromosome 7:NC_016094.2/CM000840.2; chromosome 8:NC_016095.2/CM000841.2; chromosome 9:NC_016096.2/CM000842.2; chromosome 10:NC_016097.2/CM000843.2; chromosome 11:NC_016098.2/CM000844.2; chromosome 12:NC_016099.2/CM000845.2; chromosome 13:NC_016100.2/CM000846.2; chromosome 14:NC_016101.2/CM000847.2; chromosome 15:NC_016102.2/CM000848.2; chromosome 16:NC_016103.2/CM000849.2; chromosome 17:NC_016104.2/CM000850.2; chromosome 18:NC_016105.2/CM000851.2; chromosome 19:NC_016106.2/CM000852.2; chromosome 20:NC_016107.2/CM000853.2; chloroplast Pltd:NC_007942.1/DQ317523.1; mitochondrion MT:NC_020455.1/JX463295.1\n", | |
| "5 chromosome 1:NC_015438.2/CM001064.2; chromosome 2:NC_015439.2/CM001065.2; chromosome 3:NC_015440.2/CM001066.2; chromosome 4:NC_015441.2/CM001067.2; chromosome 5:NC_015442.2/CM001068.2; chromosome 6:NC_015443.2/CM001069.2; chromosome 7:NC_015444.2/CM001070.2; chromosome 8:NC_015445.2/CM001071.2; chromosome 9:NC_015446.2/CM001072.2; chromosome 10:NC_015447.2/CM001073.2; chromosome 11:NC_015448.2/CM001074.2; chromosome 12:NC_015449.2/CM001075.2; chloroplast:NC_007898.3/DQ347959.1 \n", | |
| "6 NA \n", | |
| " WGS Scaffolds CDS Release Date\n", | |
| "1 AHAL01 7795 38554 2013-04-19 \n", | |
| "2 NA 7 48350 2001-08-13 \n", | |
| "3 APNO01 2187 57661 2011-08-12 \n", | |
| "4 ACUP02 1191 71525 2010-01-05 \n", | |
| "5 AEKE02 3224 36010 2010-12-10 \n", | |
| "6 FJWB02 72295 0 2016-08-25 \n", | |
| " GenBank FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/372/725/GCA_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0\n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/735/GCA_000001735.1_TAIR10 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/219/495/GCA_000219495.2_MedtrA17_4.0 \n", | |
| "4 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/004/515/GCA_000004515.3_Glycine_max_v2.0 \n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/188/115/GCA_000188115.2_SL2.50 \n", | |
| "6 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/075/435/GCA_900075435.2_barley_BACs_2 \n", | |
| " RefSeq FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/372/725/GCF_000372725.1_Emiliana_huxleyi_CCMP1516_main_genome_assembly_v1.0\n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/735/GCF_000001735.3_TAIR10 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/219/495/GCF_000219495.3_MedtrA17_4.0 \n", | |
| "4 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/004/515/GCF_000004515.4_Glycine_max_v2.0 \n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/188/115/GCF_000188115.3_SL2.50 \n", | |
| "6 NA " | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "# just the chromosome and scaffold level assembies, the contigs removed\n", | |
| "eukaryote = raw_eukaryote[raw_eukaryote$Level == \"Chromosome\" || raw_eukaryote$Level == \"Scaffold\",]\n", | |
| "head(eukaryote)" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "b3a8a3aa-9bbb-4cef-be9e-145006d1915d", | |
| "_uuid": "1ced7389eaf5a37e26ba58e0b99f4c760ea189d6" | |
| }, | |
| "source": [ | |
| "### read in the eukaryote data" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 4, | |
| "metadata": { | |
| "_cell_guid": "270e4339-5f7a-4837-9d8c-98436c5c3d7e", | |
| "_uuid": "3cc2aaa64f0d83df0c6f7fc9b7bd521dd7c0ecd9" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "<table>\n", | |
| "<thead><tr><th scope=col>#Organism Name</th><th scope=col>Organism Groups</th><th scope=col>Strain</th><th scope=col>BioSample</th><th scope=col>BioProject</th><th scope=col>Assembly</th><th scope=col>Level</th><th scope=col>Size(Mb)</th><th scope=col>GC%</th><th scope=col>Replicons</th><th scope=col>WGS</th><th scope=col>Scaffolds</th><th scope=col>CDS</th><th scope=col>Release Date</th><th scope=col>GenBank FTP</th><th scope=col>RefSeq FTP</th></tr></thead>\n", | |
| "<tbody>\n", | |
| "\t<tr><td>Prototheca stagnorum </td><td>Eukaryota;Plants;Green Algae </td><td>JCM 9641 </td><td>SAMD00028436 </td><td>PRJDB3715 </td><td>GCA_002794665.1 </td><td>Scaffold </td><td>16.8962 </td><td>71.4 </td><td>NA </td><td>BCJY01 </td><td>27 </td><td>0 </td><td>2017-11-16 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/794/665/GCA_002794665.1_JCM_9641_assembly_v001</td><td>NA </td></tr>\n", | |
| "</tbody>\n", | |
| "</table>\n" | |
| ], | |
| "text/latex": [ | |
| "\\begin{tabular}{r|llllllllllllllll}\n", | |
| " \\#Organism Name & Organism Groups & Strain & BioSample & BioProject & Assembly & Level & Size(Mb) & GC\\% & Replicons & WGS & Scaffolds & CDS & Release Date & GenBank FTP & RefSeq FTP\\\\\n", | |
| "\\hline\n", | |
| "\t Prototheca stagnorum & Eukaryota;Plants;Green Algae & JCM 9641 & SAMD00028436 & PRJDB3715 & GCA\\_002794665.1 & Scaffold & 16.8962 & 71.4 & NA & BCJY01 & 27 & 0 & 2017-11-16 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/794/665/GCA\\_002794665.1\\_JCM\\_9641\\_assembly\\_v001 & NA \\\\\n", | |
| "\\end{tabular}\n" | |
| ], | |
| "text/markdown": [ | |
| "\n", | |
| "#Organism Name | Organism Groups | Strain | BioSample | BioProject | Assembly | Level | Size(Mb) | GC% | Replicons | WGS | Scaffolds | CDS | Release Date | GenBank FTP | RefSeq FTP | \n", | |
| "|---|\n", | |
| "| Prototheca stagnorum | Eukaryota;Plants;Green Algae | JCM 9641 | SAMD00028436 | PRJDB3715 | GCA_002794665.1 | Scaffold | 16.8962 | 71.4 | NA | BCJY01 | 27 | 0 | 2017-11-16 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/794/665/GCA_002794665.1_JCM_9641_assembly_v001 | NA | \n", | |
| "\n", | |
| "\n" | |
| ], | |
| "text/plain": [ | |
| " #Organism Name Organism Groups Strain BioSample \n", | |
| "1 Prototheca stagnorum Eukaryota;Plants;Green Algae JCM 9641 SAMD00028436\n", | |
| " BioProject Assembly Level Size(Mb) GC% Replicons WGS Scaffolds\n", | |
| "1 PRJDB3715 GCA_002794665.1 Scaffold 16.8962 71.4 NA BCJY01 27 \n", | |
| " CDS Release Date\n", | |
| "1 0 2017-11-16 \n", | |
| " GenBank FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/794/665/GCA_002794665.1_JCM_9641_assembly_v001\n", | |
| " RefSeq FTP\n", | |
| "1 NA " | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "eukaryote = eukaryote[eukaryote$\"GC%\" > 5,]\n", | |
| "#Prototheca stagnorum has 71% GC content which is pretty neat\n", | |
| "eukaryote[eukaryote$\"GC%\" > 70,]" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 5, | |
| "metadata": { | |
| "_cell_guid": "3864b316-60e2-42da-894e-61a2780b1584", | |
| "_uuid": "d8b84411dbfec9b643975e2319bed2389c29e224" | |
| }, | |
| "outputs": [ | |
| { | |
| "name": "stderr", | |
| "output_type": "stream", | |
| "text": [ | |
| "`stat_bin()` using `bins = 30`. Pick better value with `binwidth`.\n" | |
| ] | |
| }, | |
| { | |
| "data": {}, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| }, | |
| { | |
| "data": { | |
| "image/png": 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WI/7j4ybhvx8Y9/vLf/5IJNM+U7nfYk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\n0By/1wAAAABJRU5ErkJggg==", | |
| "text/plain": [ | |
| "plot without title" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "#looking at the data there appears to be some missing data.\n", | |
| "# GC of zero seen, looks like one with with a very low number,\n", | |
| "# also an outlier with >70% gc, I should look at what this one is \n", | |
| "qplot(eukaryote$\"GC%\") +\n", | |
| " labs(title = \"Histogram of Eukaryote genome %GC content\", x= \"%GC content\", y = \"Frequency\") +\n", | |
| "\ttheme_fivethirtyeight()" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "2aba6bfd-fdb6-462a-9870-cb5fa3d80b04", | |
| "_uuid": "5a1e4c50b4ffbf5fc4718d5dcaf600ac0362b85a" | |
| }, | |
| "source": [ | |
| "### read in the prokaryote data" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 6, | |
| "metadata": { | |
| "_cell_guid": "4ed5c5c9-58a5-4ffd-81be-89082c4f3f1c", | |
| "_uuid": "e6bf165689f176a4f20298c2bc2a1857da574c15" | |
| }, | |
| "outputs": [ | |
| { | |
| "name": "stderr", | |
| "output_type": "stream", | |
| "text": [ | |
| "Parsed with column specification:\n", | |
| "cols(\n", | |
| " `#Organism Name` = col_character(),\n", | |
| " `Organism Groups` = col_character(),\n", | |
| " Strain = col_character(),\n", | |
| " BioSample = col_character(),\n", | |
| " BioProject = col_character(),\n", | |
| " Assembly = col_character(),\n", | |
| " Level = col_character(),\n", | |
| " `Size(Mb)` = col_double(),\n", | |
| " `GC%` = col_double(),\n", | |
| " Replicons = col_character(),\n", | |
| " WGS = col_character(),\n", | |
| " Scaffolds = col_integer(),\n", | |
| " CDS = col_integer(),\n", | |
| " `Release Date` = col_datetime(format = \"\"),\n", | |
| " `GenBank FTP` = col_character(),\n", | |
| " `RefSeq FTP` = col_character()\n", | |
| ")\n" | |
| ] | |
| } | |
| ], | |
| "source": [ | |
| "prokaryote = read_csv('all_genomes_lists/prokaryotes.csv')\n", | |
| "prokaryote = prokaryote[prokaryote$\"GC%\" > 5,]" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 7, | |
| "metadata": { | |
| "_cell_guid": "2eba28e4-df90-4a8b-a45d-f6dfd015cb12", | |
| "_uuid": "0d4e08e554ee613287c2265c3b56efadbca713c0" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "<table>\n", | |
| "<thead><tr><th scope=col>#Organism Name</th><th scope=col>Organism Groups</th><th scope=col>Strain</th><th scope=col>BioSample</th><th scope=col>BioProject</th><th scope=col>Assembly</th><th scope=col>Level</th><th scope=col>Size(Mb)</th><th scope=col>GC%</th><th scope=col>Replicons</th><th scope=col>WGS</th><th scope=col>Scaffolds</th><th scope=col>CDS</th><th scope=col>Release Date</th><th scope=col>GenBank FTP</th><th scope=col>RefSeq FTP</th></tr></thead>\n", | |
| "<tbody>\n", | |
| "\t<tr><td>Candidatus Carsonella ruddii PC isolate NHV </td><td>Bacteria;Proteobacteria;Gammaproteobacteria </td><td>PC </td><td>SAMN02641651 </td><td>PRJNA165191 </td><td>GCA_000287315.1 </td><td>Complete </td><td>0.159923 </td><td>15.6 </td><td>chromosome:CP003545.1 </td><td>NA </td><td>1 </td><td>182 </td><td>2012-08-13 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/287/315/GCA_000287315.1_ASM28731v1 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/287/315/GCF_000287315.1_ASM28731v1 </td></tr>\n", | |
| "\t<tr><td>Candidatus Zinderia insecticola CARI </td><td>Bacteria;Proteobacteria;Betaproteobacteria </td><td>CARI </td><td>SAMN02604227 </td><td>PRJNA51243 </td><td>GCA_000147015.1 </td><td>Complete </td><td>0.208564 </td><td>13.5 </td><td>chromosome:CP002161.1 </td><td>NA </td><td>1 </td><td>206 </td><td>2010-09-09 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/147/015/GCA_000147015.1_ASM14701v1 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/147/015/GCF_000147015.1_ASM14701v1 </td></tr>\n", | |
| "\t<tr><td>Candidatus Nasuia deltocephalinicola str. NAS-ALF </td><td>Bacteria;Proteobacteria;Betaproteobacteria </td><td>ALF </td><td>SAMN02641653 </td><td>PRJNA206993 </td><td>GCA_000442605.1 </td><td>Complete </td><td>0.112091 </td><td>17.1 </td><td>chromosome:NC_021919.1/CP006059.1 </td><td>NA </td><td>1 </td><td>138 </td><td>2013-08-01 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/442/605/GCA_000442605.1_ASM44260v1 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/442/605/GCF_000442605.1_ASM44260v1 </td></tr>\n", | |
| "\t<tr><td>Enterobacteriaceae bacterium NZ1215 </td><td>Bacteria;Proteobacteria;Gammaproteobacteria </td><td>NZ1215 </td><td>SAMN07758087 </td><td>PRJNA413589 </td><td>GCA_002688505.1 </td><td>Complete </td><td>0.271175 </td><td>15.4 </td><td>chromosome:CP024013.1 </td><td>NA </td><td>1 </td><td> 0 </td><td>2017-10-24 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/688/505/GCA_002688505.1_ASM268850v1</td><td>NA </td></tr>\n", | |
| "\t<tr><td>Candidatus Nasuia deltocephalinicola </td><td>Bacteria;Proteobacteria;Betaproteobacteria </td><td>PUNC </td><td>SAMN04223555 </td><td>PRJNA300484 </td><td>GCA_001447885.1 </td><td>Complete </td><td>0.112031 </td><td>16.6 </td><td>chromosome:NZ_CP013211.1/CP013211.1 </td><td>NA </td><td>1 </td><td>138 </td><td>2015-11-24 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/447/885/GCA_001447885.1_ASM144788v1</td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/001/447/885/GCF_001447885.1_ASM144788v1</td></tr>\n", | |
| "\t<tr><td>Candidatus Carsonella ruddii PV </td><td>Bacteria;Proteobacteria;Gammaproteobacteria </td><td>PV </td><td>SAMD00061085 </td><td>PRJNA17977 </td><td>GCA_000010365.1 </td><td>Complete </td><td>0.159662 </td><td>16.6 </td><td>chromosome:AP009180.1 </td><td>NA </td><td>1 </td><td>182 </td><td>2006-10-17 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/010/365/GCA_000010365.1_ASM1036v1 </td><td>ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/010/365/GCF_000010365.1_ASM1036v1 </td></tr>\n", | |
| "</tbody>\n", | |
| "</table>\n" | |
| ], | |
| "text/latex": [ | |
| "\\begin{tabular}{r|llllllllllllllll}\n", | |
| " \\#Organism Name & Organism Groups & Strain & BioSample & BioProject & Assembly & Level & Size(Mb) & GC\\% & Replicons & WGS & Scaffolds & CDS & Release Date & GenBank FTP & RefSeq FTP\\\\\n", | |
| "\\hline\n", | |
| "\t Candidatus Carsonella ruddii PC isolate NHV & Bacteria;Proteobacteria;Gammaproteobacteria & PC & SAMN02641651 & PRJNA165191 & GCA\\_000287315.1 & Complete & 0.159923 & 15.6 & chromosome:CP003545.1 & NA & 1 & 182 & 2012-08-13 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/287/315/GCA\\_000287315.1\\_ASM28731v1 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/287/315/GCF\\_000287315.1\\_ASM28731v1 \\\\\n", | |
| "\t Candidatus Zinderia insecticola CARI & Bacteria;Proteobacteria;Betaproteobacteria & CARI & SAMN02604227 & PRJNA51243 & GCA\\_000147015.1 & Complete & 0.208564 & 13.5 & chromosome:CP002161.1 & NA & 1 & 206 & 2010-09-09 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/147/015/GCA\\_000147015.1\\_ASM14701v1 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/147/015/GCF\\_000147015.1\\_ASM14701v1 \\\\\n", | |
| "\t Candidatus Nasuia deltocephalinicola str. NAS-ALF & Bacteria;Proteobacteria;Betaproteobacteria & ALF & SAMN02641653 & PRJNA206993 & GCA\\_000442605.1 & Complete & 0.112091 & 17.1 & chromosome:NC\\_021919.1/CP006059.1 & NA & 1 & 138 & 2013-08-01 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/442/605/GCA\\_000442605.1\\_ASM44260v1 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/442/605/GCF\\_000442605.1\\_ASM44260v1 \\\\\n", | |
| "\t Enterobacteriaceae bacterium NZ1215 & Bacteria;Proteobacteria;Gammaproteobacteria & NZ1215 & SAMN07758087 & PRJNA413589 & GCA\\_002688505.1 & Complete & 0.271175 & 15.4 & chromosome:CP024013.1 & NA & 1 & 0 & 2017-10-24 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/688/505/GCA\\_002688505.1\\_ASM268850v1 & NA \\\\\n", | |
| "\t Candidatus Nasuia deltocephalinicola & Bacteria;Proteobacteria;Betaproteobacteria & PUNC & SAMN04223555 & PRJNA300484 & GCA\\_001447885.1 & Complete & 0.112031 & 16.6 & chromosome:NZ\\_CP013211.1/CP013211.1 & NA & 1 & 138 & 2015-11-24 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/447/885/GCA\\_001447885.1\\_ASM144788v1 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/001/447/885/GCF\\_001447885.1\\_ASM144788v1\\\\\n", | |
| "\t Candidatus Carsonella ruddii PV & Bacteria;Proteobacteria;Gammaproteobacteria & PV & SAMD00061085 & PRJNA17977 & GCA\\_000010365.1 & Complete & 0.159662 & 16.6 & chromosome:AP009180.1 & NA & 1 & 182 & 2006-10-17 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/010/365/GCA\\_000010365.1\\_ASM1036v1 & ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/010/365/GCF\\_000010365.1\\_ASM1036v1 \\\\\n", | |
| "\\end{tabular}\n" | |
| ], | |
| "text/markdown": [ | |
| "\n", | |
| "#Organism Name | Organism Groups | Strain | BioSample | BioProject | Assembly | Level | Size(Mb) | GC% | Replicons | WGS | Scaffolds | CDS | Release Date | GenBank FTP | RefSeq FTP | \n", | |
| "|---|---|---|---|---|---|\n", | |
| "| Candidatus Carsonella ruddii PC isolate NHV | Bacteria;Proteobacteria;Gammaproteobacteria | PC | SAMN02641651 | PRJNA165191 | GCA_000287315.1 | Complete | 0.159923 | 15.6 | chromosome:CP003545.1 | NA | 1 | 182 | 2012-08-13 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/287/315/GCA_000287315.1_ASM28731v1 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/287/315/GCF_000287315.1_ASM28731v1 | \n", | |
| "| Candidatus Zinderia insecticola CARI | Bacteria;Proteobacteria;Betaproteobacteria | CARI | SAMN02604227 | PRJNA51243 | GCA_000147015.1 | Complete | 0.208564 | 13.5 | chromosome:CP002161.1 | NA | 1 | 206 | 2010-09-09 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/147/015/GCA_000147015.1_ASM14701v1 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/147/015/GCF_000147015.1_ASM14701v1 | \n", | |
| "| Candidatus Nasuia deltocephalinicola str. NAS-ALF | Bacteria;Proteobacteria;Betaproteobacteria | ALF | SAMN02641653 | PRJNA206993 | GCA_000442605.1 | Complete | 0.112091 | 17.1 | chromosome:NC_021919.1/CP006059.1 | NA | 1 | 138 | 2013-08-01 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/442/605/GCA_000442605.1_ASM44260v1 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/442/605/GCF_000442605.1_ASM44260v1 | \n", | |
| "| Enterobacteriaceae bacterium NZ1215 | Bacteria;Proteobacteria;Gammaproteobacteria | NZ1215 | SAMN07758087 | PRJNA413589 | GCA_002688505.1 | Complete | 0.271175 | 15.4 | chromosome:CP024013.1 | NA | 1 | 0 | 2017-10-24 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/688/505/GCA_002688505.1_ASM268850v1 | NA | \n", | |
| "| Candidatus Nasuia deltocephalinicola | Bacteria;Proteobacteria;Betaproteobacteria | PUNC | SAMN04223555 | PRJNA300484 | GCA_001447885.1 | Complete | 0.112031 | 16.6 | chromosome:NZ_CP013211.1/CP013211.1 | NA | 1 | 138 | 2015-11-24 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/447/885/GCA_001447885.1_ASM144788v1 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/001/447/885/GCF_001447885.1_ASM144788v1 | \n", | |
| "| Candidatus Carsonella ruddii PV | Bacteria;Proteobacteria;Gammaproteobacteria | PV | SAMD00061085 | PRJNA17977 | GCA_000010365.1 | Complete | 0.159662 | 16.6 | chromosome:AP009180.1 | NA | 1 | 182 | 2006-10-17 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/010/365/GCA_000010365.1_ASM1036v1 | ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/010/365/GCF_000010365.1_ASM1036v1 | \n", | |
| "\n", | |
| "\n" | |
| ], | |
| "text/plain": [ | |
| " #Organism Name \n", | |
| "1 Candidatus Carsonella ruddii PC isolate NHV \n", | |
| "2 Candidatus Zinderia insecticola CARI \n", | |
| "3 Candidatus Nasuia deltocephalinicola str. NAS-ALF\n", | |
| "4 Enterobacteriaceae bacterium NZ1215 \n", | |
| "5 Candidatus Nasuia deltocephalinicola \n", | |
| "6 Candidatus Carsonella ruddii PV \n", | |
| " Organism Groups Strain BioSample BioProject \n", | |
| "1 Bacteria;Proteobacteria;Gammaproteobacteria PC SAMN02641651 PRJNA165191\n", | |
| "2 Bacteria;Proteobacteria;Betaproteobacteria CARI SAMN02604227 PRJNA51243 \n", | |
| "3 Bacteria;Proteobacteria;Betaproteobacteria ALF SAMN02641653 PRJNA206993\n", | |
| "4 Bacteria;Proteobacteria;Gammaproteobacteria NZ1215 SAMN07758087 PRJNA413589\n", | |
| "5 Bacteria;Proteobacteria;Betaproteobacteria PUNC SAMN04223555 PRJNA300484\n", | |
| "6 Bacteria;Proteobacteria;Gammaproteobacteria PV SAMD00061085 PRJNA17977 \n", | |
| " Assembly Level Size(Mb) GC% Replicons \n", | |
| "1 GCA_000287315.1 Complete 0.159923 15.6 chromosome:CP003545.1 \n", | |
| "2 GCA_000147015.1 Complete 0.208564 13.5 chromosome:CP002161.1 \n", | |
| "3 GCA_000442605.1 Complete 0.112091 17.1 chromosome:NC_021919.1/CP006059.1 \n", | |
| "4 GCA_002688505.1 Complete 0.271175 15.4 chromosome:CP024013.1 \n", | |
| "5 GCA_001447885.1 Complete 0.112031 16.6 chromosome:NZ_CP013211.1/CP013211.1\n", | |
| "6 GCA_000010365.1 Complete 0.159662 16.6 chromosome:AP009180.1 \n", | |
| " WGS Scaffolds CDS Release Date\n", | |
| "1 NA 1 182 2012-08-13 \n", | |
| "2 NA 1 206 2010-09-09 \n", | |
| "3 NA 1 138 2013-08-01 \n", | |
| "4 NA 1 0 2017-10-24 \n", | |
| "5 NA 1 138 2015-11-24 \n", | |
| "6 NA 1 182 2006-10-17 \n", | |
| " GenBank FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/287/315/GCA_000287315.1_ASM28731v1 \n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/147/015/GCA_000147015.1_ASM14701v1 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/442/605/GCA_000442605.1_ASM44260v1 \n", | |
| "4 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/002/688/505/GCA_002688505.1_ASM268850v1\n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/447/885/GCA_001447885.1_ASM144788v1\n", | |
| "6 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/010/365/GCA_000010365.1_ASM1036v1 \n", | |
| " RefSeq FTP \n", | |
| "1 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/287/315/GCF_000287315.1_ASM28731v1 \n", | |
| "2 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/147/015/GCF_000147015.1_ASM14701v1 \n", | |
| "3 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/442/605/GCF_000442605.1_ASM44260v1 \n", | |
| "4 NA \n", | |
| "5 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/001/447/885/GCF_001447885.1_ASM144788v1\n", | |
| "6 ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/010/365/GCF_000010365.1_ASM1036v1 " | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "#check to make sure things aren't valuable\n", | |
| "low_prokaryote = prokaryote[prokaryote$\"GC%\" < 20,]\n", | |
| "head(low_prokaryote)" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 8, | |
| "metadata": { | |
| "_cell_guid": "c1f7a396-4108-4fe0-855d-c440680b05e3", | |
| "_uuid": "51a02806f5eb302ff0fcb665b7e5f7cca485818c", | |
| "scrolled": true | |
| }, | |
| "outputs": [ | |
| { | |
| "name": "stderr", | |
| "output_type": "stream", | |
| "text": [ | |
| "`stat_bin()` using `bins = 30`. Pick better value with `binwidth`.\n" | |
| ] | |
| }, | |
| { | |
| "data": {}, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| }, | |
| { | |
| "data": { | |
| "image/png": 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V0r5URqkgApy01XjOzAy8Govq7s9NPNJv9OP/30XqtSWmty8B03NXd86/WJHvRd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\newAAAABJRU5ErkJggg==", | |
| "text/plain": [ | |
| "plot without title" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "#wider distribution for prokaryotes\n", | |
| "# higher mean and median\n", | |
| "qplot(prokaryote$\"GC%\") +\n", | |
| " labs(title = \"Histogram of Prokaryote genome %GC content\", x= \"%GC content\", y = \"Frequency\") +\n", | |
| "\ttheme_fivethirtyeight()" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "5886bde7-b385-4f22-ac1e-a23698b0afba", | |
| "_uuid": "9d28be227722bf3805116622a3ad7900a7e98bcc" | |
| }, | |
| "source": [ | |
| "### read in the virus data" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 9, | |
| "metadata": { | |
| "_cell_guid": "c53fde9d-e318-45b1-be69-3b5b4fa6ad42", | |
| "_uuid": "a85f4af09f7ad423f8c4df70029149c5a353168c" | |
| }, | |
| "outputs": [ | |
| { | |
| "name": "stderr", | |
| "output_type": "stream", | |
| "text": [ | |
| "Warning message:\n", | |
| "“Duplicated column names deduplicated: 'Replicons' => 'Replicons_1' [16]”Parsed with column specification:\n", | |
| "cols(\n", | |
| " `#Organism Name` = col_character(),\n", | |
| " `Organism Groups` = col_character(),\n", | |
| " BioSample = col_character(),\n", | |
| " BioProject = col_character(),\n", | |
| " Assembly = col_character(),\n", | |
| " Level = col_character(),\n", | |
| " `Size(Mb)` = col_double(),\n", | |
| " `GC%` = col_double(),\n", | |
| " Replicons = col_character(),\n", | |
| " Host = col_character(),\n", | |
| " CDS = col_integer(),\n", | |
| " Neighbors = col_integer(),\n", | |
| " `Release Date` = col_datetime(format = \"\"),\n", | |
| " `GenBank FTP` = col_character(),\n", | |
| " `RefSeq FTP` = col_character(),\n", | |
| " Replicons_1 = col_character()\n", | |
| ")\n" | |
| ] | |
| } | |
| ], | |
| "source": [ | |
| "#one 0 in the viruses... also a gc as high as 80+ investigate further\n", | |
| "viruses = read_csv('all_genomes_lists/viruses.csv')\n", | |
| "viruses = viruses[viruses$\"GC%\" > 5,]" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 10, | |
| "metadata": { | |
| "_cell_guid": "1455fc63-bece-4a17-abc3-1f9625067504", | |
| "_uuid": "a4f5e8a265b5d0bfd32db4a7d48e7090c1393dfa" | |
| }, | |
| "outputs": [ | |
| { | |
| "name": "stderr", | |
| "output_type": "stream", | |
| "text": [ | |
| "`stat_bin()` using `bins = 30`. Pick better value with `binwidth`.\n" | |
| ] | |
| }, | |
| { | |
| "data": {}, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| }, | |
| { | |
| "data": { | |
| "image/png": 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63j0yrUT5DySO5cMS1PBHL/RdkldK3zXc46sNZ5JPnLBYf33vPQj1lDEoT8y5Mj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\nW14JECBAgAABAgQIEKgoIECqyGMjAQIECBAgQIA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| |
| "text/plain": [ | |
| "plot without title" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "qplot(viruses$\"GC%\")+\n", | |
| " labs(title = \"Histogram of Virus genome %GC content\", x= \"%GC content\", y = \"Frequency\") +\n", | |
| "\ttheme_fivethirtyeight()" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "af565c22-10aa-4a2d-9b06-8749ffd56a53", | |
| "_uuid": "84ad6c4779e2b152ef5a99e001980ba6cbce816f" | |
| }, | |
| "source": [ | |
| "## %GC content - comparison of means and medians\n", | |
| "\n", | |
| "As a first step we can assess the means and medians for the different organism types." | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 11, | |
| "metadata": { | |
| "_cell_guid": "00d6e0ae-794d-4c48-b6ba-2905cbe5facb", | |
| "_uuid": "78d41c0648efbdaa834920a239eaae09264a9de0" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "43.1458628725745" | |
| ], | |
| "text/latex": [ | |
| "43.1458628725745" | |
| ], | |
| "text/markdown": [ | |
| "43.1458628725745" | |
| ], | |
| "text/plain": [ | |
| "[1] 43.14586" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "mean(eukaryote$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 12, | |
| "metadata": { | |
| "_cell_guid": "29847cea-ab44-4673-825a-756fe27362ad", | |
| "_uuid": "a596b7a311ccaaaa7bb23843fe93dcfea88e34e5" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "49.4056430238784" | |
| ], | |
| "text/latex": [ | |
| "49.4056430238784" | |
| ], | |
| "text/markdown": [ | |
| "49.4056430238784" | |
| ], | |
| "text/plain": [ | |
| "[1] 49.40564" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "mean(prokaryote$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 13, | |
| "metadata": { | |
| "_cell_guid": "c14bb67f-db6d-4f4d-a91e-bf3a4a2e2848", | |
| "_uuid": "8cfe7f480b61125781f799f0652be6ee51e21fb0" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "43.2361997418829" | |
| ], | |
| "text/latex": [ | |
| "43.2361997418829" | |
| ], | |
| "text/markdown": [ | |
| "43.2361997418829" | |
| ], | |
| "text/plain": [ | |
| "[1] 43.2362" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "#mean of this GC in between the two others\n", | |
| "mean(viruses$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 14, | |
| "metadata": { | |
| "_cell_guid": "215becea-a051-40d6-9ff9-d3173818e9f2", | |
| "_uuid": "863ebde74c52d3e1b7d1ee51eca29f97004a557d" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "42.3" | |
| ], | |
| "text/latex": [ | |
| "42.3" | |
| ], | |
| "text/markdown": [ | |
| "42.3" | |
| ], | |
| "text/plain": [ | |
| "[1] 42.3" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "median(eukaryote$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 15, | |
| "metadata": { | |
| "_cell_guid": "38846277-2402-44b7-917d-5b605570f952", | |
| "_uuid": "283565f4be167094ae90812a01c9d66f4016473a" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "50.6" | |
| ], | |
| "text/latex": [ | |
| "50.6" | |
| ], | |
| "text/markdown": [ | |
| "50.6" | |
| ], | |
| "text/plain": [ | |
| "[1] 50.6" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "median(prokaryote$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 16, | |
| "metadata": { | |
| "_cell_guid": "04835b3a-4eaa-4efd-ab5e-df7ebfe9e69a", | |
| "_uuid": "dceb4ac722088fc10011e0cc5f61fd2220628ab1" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
| "text/html": [ | |
| "39.5" | |
| ], | |
| "text/latex": [ | |
| "39.5" | |
| ], | |
| "text/markdown": [ | |
| "39.5" | |
| ], | |
| "text/plain": [ | |
| "[1] 39.5" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "median(viruses$\"GC%\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "770eda53-f599-440b-b7ae-735a57a8afa8", | |
| "_uuid": "3fce1560d8417542e8a21b5143f8e5212a7a2e2e" | |
| }, | |
| "source": [ | |
| "## Comparing trends in the genomes of Viruses, Prokaryotes and Eukaryotes\n", | |
| "\n", | |
| "Here I put all three organism types into a single dataframe for comparing the GC content and genome size (mb) across the domains." | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 17, | |
| "metadata": { | |
| "_cell_guid": "9df01aaa-a21c-407d-a8a1-69eda9b5ad15", | |
| "_uuid": "ac36fac123ce3e268f6f11aa9b8dd2af5470fdb9" | |
| }, | |
| "outputs": [], | |
| "source": [ | |
| "all_genomes = data.frame(domain= \"virus\", \n", | |
| "\t\t\t\t\t\t\tGC = viruses$\"GC%\", \n", | |
| "\t\t\t\t\t\t\tsize_mb = viruses$\"Size(Mb)\" )\n", | |
| "\n", | |
| "all_genomes = rbind(all_genomes, data.frame(domain=\"prokaryote\", \n", | |
| "\t\t\t\t\t\t\t\t\t\t\t\tGC = prokaryote$\"GC%\", \n", | |
| "\t\t\t\t\t\t\t\t\t\t\t\tsize_mb = prokaryote$\"Size(Mb)\"))\n", | |
| "all_genomes = rbind(all_genomes, data.frame(domain=\"eukaryote\", \n", | |
| "\t\t\t\t\t\t\t\t\t\t\t\tGC = eukaryote$\"GC%\", \n", | |
| "\t\t\t\t\t\t\t\t\t\t\t\tsize_mb = eukaryote$\"Size(Mb)\"))" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "cc0b61c8-6a92-4a94-a7d4-7767d2283431", | |
| "_uuid": "543be8a8a2e027a9af52d9c1e31861adaa6d7a31" | |
| }, | |
| "source": [ | |
| "First a simple boxplot of the gc content across the categories" | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 18, | |
| "metadata": { | |
| "_cell_guid": "f7525d8f-f692-4bdf-be77-9b4542ab1d82", | |
| "_uuid": "983c7f277f292e5db62d2865a93264183ce17d96" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": { | |
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L2f2E5WwnnSUxu5k9PFLn9U0vt7eZ7i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\n07iO3vUaJ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LYztpXdEEAAAQQQQAABBBAorUDdB0gB3r9kt0+y4HeN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\nTkSuQmCC", | |
| "text/plain": [ | |
| "Plot with title “%GC content of sequenced Virus, Prokaryote and Eukaryote genomes”" | |
| ] | |
| }, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| } | |
| ], | |
| "source": [ | |
| "boxplot(GC ~ domain , data = all_genomes,\n", | |
| " main = \"%GC content of sequenced Virus, Prokaryote and Eukaryote genomes\")" | |
| ] | |
| }, | |
| { | |
| "cell_type": "markdown", | |
| "metadata": { | |
| "_cell_guid": "69094e76-2c25-4698-880f-e467c645645e", | |
| "_uuid": "819f081ef4c7cefd2198faa3b1b2f1ee1a7539fd" | |
| }, | |
| "source": [ | |
| "I find that by overlaying a scatter plot on top of the boxplot, we can visualize the distribution of the data more effectively. Below I accomplish this using ggplot to produce a boxplt via `geom_boxplot` and then I overlay translucent, jittered (moved to not overlap to heavily) dots via `geom_point`. The rest of the lines are visual blandishment." | |
| ] | |
| }, | |
| { | |
| "cell_type": "code", | |
| "execution_count": 19, | |
| "metadata": { | |
| "_cell_guid": "caf9a337-70c3-4c77-8f9e-63ffc8931a89", | |
| "_uuid": "f91c506a2b8ec9c5fce1026ebe03dd01e4ed04ba" | |
| }, | |
| "outputs": [ | |
| { | |
| "data": {}, | |
| "metadata": {}, | |
| "output_type": "display_data" | |
| }, | |
| { | |
| "data": { |
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