Created
September 5, 2024 14:05
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Orthoslicer in Python
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def orthoslicer(stack, direction, coordinates, verbose=False): | |
'''Works like "orthogonal view" (CTRL+Shift+H in ImageJ''' | |
if verbose: | |
print('The input stack has a size of %s x %s x %s px' % (stack.shape[0], | |
stack.shape[1], | |
stack.shape[2])) | |
print('Extracting a slice centered %s, %s, %s px in direction %s' % (coordinates[0], | |
coordinates[1], | |
coordinates[2], | |
direction)) | |
if direction == 0: | |
if verbose: | |
print('Extracting YZ slice %s of %s' % (coordinates[0], stack.shape[2])) | |
extractedslice = dask.array.rot90(stack[:, :, coordinates[0]]) | |
elif direction == 1: | |
if verbose: | |
print('Extracting XZ slice %s of %s' % (coordinates[1], stack.shape[1])) | |
extractedslice = stack[:,coordinates[1],:] | |
elif direction == 2: | |
if verbose: | |
print('Extracting slice %s of %s' % (coordinates[2], stack.shape[0])) | |
extractedslice = stack[coordinates[2],:,:] | |
if verbose: | |
plt.figure(999) | |
for anatomical_direction in range(3): | |
if anatomical_direction == 0: | |
plt.subplot(2,2,2) | |
# Flip and rotate the extracted YZ slice, to be exactly consistent with the OrthoViewer of ImageJ | |
plt.imshow(skimage.exposure.equalize_adapthist(dask.array.flipud(dask.array.rot90(stack[:, :, coordinates[0]])))) | |
plt.axhline(coordinates[1], c=seaborn.color_palette(n_colors=3)[1]) | |
plt.axvline(coordinates[2], c=seaborn.color_palette(n_colors=3)[2]) | |
plt.title('YZ slice %s (dir=%s)\nCoords: %s' % (coordinates[0], anatomical_direction, coordinates), color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
plt.gca().tick_params(axis='both', color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
elif anatomical_direction == 1: | |
plt.subplot(2,2,3) | |
plt.imshow(skimage.exposure.equalize_adapthist(stack[:,coordinates[1],:])) | |
plt.axhline(coordinates[2], c=seaborn.color_palette(n_colors=3)[2]) | |
plt.axvline(coordinates[0], c=seaborn.color_palette(n_colors=3)[0]) | |
plt.title('XZ slice %s (dir=%s)\nCoords: %s' % (coordinates[1], anatomical_direction, coordinates), color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
plt.gca().tick_params(axis='both', color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
elif anatomical_direction == 2: | |
plt.subplot(2,2,1) | |
plt.imshow(skimage.exposure.equalize_adapthist(stack[coordinates[2],:,:])) | |
plt.axhline(coordinates[1], c=seaborn.color_palette(n_colors=3)[1]) | |
plt.axvline(coordinates[0], c=seaborn.color_palette(n_colors=3)[0]) | |
plt.title('Slice %s (dir=%s)\nCoords: %s' % (coordinates[2], anatomical_direction, coordinates), color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
plt.gca().tick_params(axis='both', color=seaborn.color_palette(n_colors=3)[anatomical_direction]) | |
plt.tight_layout(pad=0.1) | |
plt.show() | |
return(extractedslice) |
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# Test extraction script | |
# Coordinates are set at the border of left big blood vessel on slice 2434 on 11um stack of d152, as shown in OrthoViewer of ImageJ | |
extracted_slice = orthoslicer(Reconstructions[15], | |
2, | |
(1296,1784,2600), | |
verbose=True) | |
plt.figure() | |
plt.imshow(extracted_slice) | |
plt.show() |
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My code on the left, screenshot of ImageJ on the right.
