Before using this for samples that are precious, you should thoroughly test the solution that you make. The best way to do that would be a side-by-side with commercial RNALater.
The following recipe is from the patent. It always pays to read these.
In a beaker, combine 40 ml 0.5 M EDTA, 25 ml 1M Sodium Citrate, 700 gm Ammonium Sulfate
| #' @title PRESS | |
| #' @author Thomas Hopper | |
| #' @description Returns the PRESS statistic (predictive residual sum of squares). | |
| #' Useful for evaluating predictive power of regression models. | |
| #' @param linear.model A linear regression model (class 'lm'). Required. | |
| #' | |
| PRESS <- function(linear.model) { | |
| #' calculate the predictive residuals | |
| pr <- residuals(linear.model)/(1-lm.influence(linear.model)$hat) | |
| #' calculate the PRESS |
| #!/usr/bin/perl | |
| # Description: This script converts bedGraph to fixedStep wig format with defined step size. Input file may be compressed as .gz. | |
| # Coordinates in bedGraph input are assumed to be 0-based (http://genome.ucsc.edu/goldenPath/help/bedgraph.html). | |
| # Coordinates in wig output are 1-based (http://genome.ucsc.edu/goldenPath/help/wiggle.html). | |
| # Usage: bedgraph_to_wig.pl --bedgraph input.bedgraph --wig output.wig --step step_size [--compact] | |
| # --bedgraph : specify input file in bedGraph format. | |
| # --wig : specify output file in fixedStep format. | |
| # --step : specify step size. Note that span is set to be identical to step. |
| This post examines the features of [R Markdown](http://www.rstudio.org/docs/authoring/using_markdown) | |
| using [knitr](http://yihui.name/knitr/) in Rstudio 0.96. | |
| This combination of tools provides an exciting improvement in usability for | |
| [reproducible analysis](http://stats.stackexchange.com/a/15006/183). | |
| Specifically, this post | |
| (1) discusses getting started with R Markdown and `knitr` in Rstudio 0.96; | |
| (2) provides a basic example of producing console output and plots using R Markdown; | |
| (3) highlights several code chunk options such as caching and controlling how input and output is displayed; | |
| (4) demonstrates use of standard Markdown notation as well as the extended features of formulas and tables; and | |
| (5) discusses the implications of R Markdown. |
